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Fig. 5 | Irish Veterinary Journal

Fig. 5

From: Organic acids mitigate Streptococcus agalactiae virulence in Tilapia fish gut primary cells and in a gut infection model

Fig. 5

The impact of Aq on the expression of MCP-8 and Duo-1 in the in vitro Tilapia gut model. Panel A – experimental diagram. Panel B – MCP-8 and Duo-1 gene expression in the S. agalactiae infected tissue in the presence of 0.125% Aq or in tissue infected with Aq pre-treated S. agalactiae. Gene expression data are expressed as 2−ΔΔCt; values are the mean of three test replicates relative to the reference gene 16 S RNA ribosomal subunit. The infection data is presented as CFU/ml lysate. The Student’s t-test was used to statistically compare the effect of AuraAqua. P values are indicated on the graphs. Experimental designed was produced using Biorender.com

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