Fig. 6

Modulation of the ATG7-LC3 Autophagy Pathway by bta-miR-221 Mimics. A, The initial localization of GFP-LC3 and mCherry-ATG7 in 293T cells was observed via confocal microscopy, establishing baseline cellular distribution patterns of these autophagy markers. B, Examination of the impact of bta-miR-221 mimics and inhibitors on the autophagy pathway. The bta-miR-221 mimics or inhibitors (and their respective controls) were cotransfected with GFP-LC3 and mCherry-ATG7 in HEK-293T cells. Cellular localization and interaction patterns were observed through confocal microscopy imaging 24 h posttransfection. C, HEK-293T cells were transfected with combinations of GFP-LC3 + mCherry-ATG7 and GFP-LC3 + vector-mCherry, followed by immunoprecipitation to analyse the association between the GFP and mCherry proteins, as quantified by Western blotting