Table 1 Sequences of the primers used for MicroRNA quantification
Name of Gene | Primer Sequence(5’-3’) |
|---|---|
bta-miR-2887 | F: CGCGGGACCGGGGTCC |
R: AGTGCAGGGTCCGAGGTATT | |
bta-miR-2892 | F: GGCGACGGAGGCGCGA |
R: AGTGCAGGGTCCGAGGTATT | |
bta-miR-2424 | F: CGCGCAGATCTTTGGTAATCT |
R: AGTGCAGGGTCCGAGGTATT | |
bta-miR-223 | F: GCGCGTGTCAGTTTGTCAAAT |
R: AGTGCAGGGTCCGAGGTATT | |
bta-miR-135b | F: CGCGTATGGCTTTTCATTCCT |
R: AGTGCAGGGTCCGAGGTATT | |
bta-miR-2332 | F: CGCGGTTTAAGGTCTTGGAG |
R: AGTGCAGGGTCCGAGGTATT | |
bta-miR-7 | F: CGCGTGGAAGACTAGTGATTTT |
R: AGTGCAGGGTCCGAGGTATT | |
bta-miR-3600 | F: CGCGACAGTTCTTCAACTGG |
R: AGTGCAGGGTCCGAGGTATT | |
bta-miR-378 | F: CGCGACTGGACTTGGAGTCA |
R: AGTGCAGGGTCCGAGGTATT | |
bta-miR-221 | F: CGCGAGCTACATTGTCTGCT |
R: AGTGCAGGGTCCGAGGTATT | |
bta-miR-222 | F: GCGCGAGCTACATCTGGCTA |
R: AGTGCAGGGTCCGAGGTATT | |
bta-miR-26c | F: CGCGAGCCTATCCTGGATTA |
R: AGTGCAGGGTCCGAGGTATT | |
bta-miR-221 stem‒loop sequence | 5’GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAAACCC-3’ |
GAPDH | F: TGCACCACCAACTGCTTAG |
R: GATGCAGGGATGATGTTC |