Fig. 4

Effect of transport group (IRE, INT, NLD) on immune cell subsets (white blood cell, neutrophil lymphocyte, and monocyte counts) and immunoglobulins (A, G, and M) (mean ± CI)) on arrival and the interaction of group and time (1WK: 1 week post arrival, 3WK: 3 week post arrival) on the recovery of these blood variables. Values for arrival and subsequent recovery were analysed in 2 different statistical models. Subscripts highlight significant (p < 0.05) differences between IRE and INT (a), between INT and NLD (b), or between IRE and NLD (c) calves within a timepoint. Shaded areas represent areas outside (red/dark) or inside (green/light) reference limits (based on previous research [25]) except for immunoglobulins G, M, and A, for which no reference limits were available