Fig. 3

Disagnostic PCR for some bacterial diseases causing pneumonia in sheep. Agarose gel electrophoresis of the group-specific primer set of the A phoA gene (genetic marker of E. coli), with the expected band size of 720 bp. B P. multocida Kmt1 target gene at 460 bp. C Group-specific primer set of Mannheimia haemolytica ssa at the expected band size of 508 bp. D Group-specific primer set for Mycoplasma16S rRNA, with the target gene at 280 bp. E The group-specific primer set for Streptococcus16S rRNA, with positive samples except the 912 bp Lane (M): DNA ladder. Lane (N): negative control. Lane (P): control positive Lanes (1--10) samples