Single nucleotide polymorphisms, gene expression and evaluation of immunological, antioxidant, and pathological parameters associated with bacterial pneumonia in Barki sheep

Sayed, Ahmed El; Hafez, Amani; Ateya, Ahmed; Darwish, Asmaa; Tahoun, Amin

doi:10.1186/s13620-025-00296-1

Irish Veterinary Journal

Table 1 Primer sequences, target genes, amplicon sizes and cycling conditions for bacteria and some virulence genes

From: Single nucleotide polymorphisms, gene expression and evaluation of immunological, antioxidant, and pathological parameters associated with bacterial pneumonia in Barki sheep

Target gene	Primers sequences	Amplified segment (bp)	Primary denaturation	Amplification (35 cycles)			Final extension	Reference
Target gene	Primers sequences	Amplified segment (bp)	Primary denaturation	Secondary denaturation	Annealing	Extension	Final extension	Reference
*Klebsiella. pneumoniae 16–23 S ITS*	ATTTGAAGAGGTTGCAAACGAT	130	94˚C 5 min	94˚C 30 s.	55˚C 30 s	0.72˚C 30 s.	72˚C 7 min	Turton et al. 2010 [44]
*Klebsiella. pneumoniae 16–23 S ITS*	TTCACTCTGAAGTTTTCTTGTGTTC	130	94˚C 5 min	94˚C 30 s.	55˚C 30 s	0.72˚C 30 s.	72˚C 7 min	Turton et al. 2010 [44]
*Klebsiella iutA*	GGCTGGACATGGGAACTGG	300	94˚C 5 min	94˚C 30 s.	63˚C 30 s.	72˚C 30 s.	72˚C 7 min	Yaguchi et al. 2007 [28]
*Klebsiella iutA*	CGTCGGGAACGGGTAGAATCG	300	94˚C 5 min	94˚C 30 s.	63˚C 30 s.	72˚C 30 s.	72˚C 7 min	Yaguchi et al. 2007 [28]
*Klebsiella* fimH	TGCAGAACGGATAAGCCGTGG	508	94˚C 5 min.	94˚C 30 s.	50˚C 40 s	72˚C 45 s	72˚C 10 min	Ghanbarpour and Salehi, 2010 [45]
	GCAGTCACCTGCCCTCCGGTA
	GTAAATGCACTTGCTTCAGGAC
*Pseudomonas 16 S rDNA*	GACGGGTGAGTAATGCCTA	618	94˚C 5 min.	94˚C 30 s.	50˚C 40 s.	72˚C 45 s	72˚C 10 min.	Spilker et al. 2004 [48]
	CACTGGTGTTCCTTCCTATA
	CGCTGGCCCATTCGCTCCAGCGCT
*E. coli phoA*	CGATTCTGGAAATGGCAAAAG	720	94˚C 5 min	94˚C 30 s.	55˚C 40 s	72˚C 45 s	. 72˚C 10 min	Hu et al. 2011 [52]
	CGTGATCAGCGGTGACTATGAC
	ccggccatattcacataa
*P. multocida* *Kmt1*	ATCCGCTATTTACCCAGTGG	460	94˚C 5 min.	94˚C 30 s.	55˚C 40 s	72˚C 45 s.	72˚C 10 min.	Duan et al. 2024 [46]
*P. multocida* *Kmt1*	GCTGTAAACGAACTCGCCAC	460	94˚C 5 min.	94˚C 30 s.	55˚C 40 s	72˚C 45 s.	72˚C 10 min.	Duan et al. 2024 [46]
*Mannheimia haemolytica ssa*	TTCACATCTTCATCCTC	325	94˚C 5 min.	94˚C 30 s.	50˚C 40 s.	72˚C 40 s.	72˚C 10 min	Hawari et al. 2008 [47]
*Mannheimia haemolytica ssa*	TTTTCATCCTCTTCGTC	325	94˚C 5 min.	94˚C 30 s.	50˚C 40 s.	72˚C 40 s.	72˚C 10 min	Hawari et al. 2008 [47]
Mycoplasma *16 S rRNA*	GGGAGCAAACAGGATTAGATACCCT	280	94˚C 5 min	94˚C 30 s.	55˚C 30 s.	72˚C 30 s.	72˚C 7 min.	Van Kuppeveld et al. 1994 [50]
Mycoplasma *16 S rRNA*	TGCACCATCTGTCACTCTGTTAACCTC	280	94˚C 5 min	94˚C 30 s.	55˚C 30 s.	72˚C 30 s.	72˚C 7 min.	Van Kuppeveld et al. 1994 [50]
Streptococcus *16 S rRNA*	CGGGGGATAACTATTGGAAACGATA	912		94˚C 30 s.	55˚C 40 s.	72˚C 1 min.	72˚C 10 min.	Osakabe et al. 2006 [51]
Streptococcus *16 S rRNA*	ACCTGTCACCCGATGTACCGAAGTA	912		94˚C 30 s.	55˚C 40 s.	72˚C 1 min.	72˚C 10 min.	Osakabe et al. 2006 [51]

Back to article page

ISSN: 2046-0481

Contact us

General enquiries: journalsubmissions@springernature.com