Table 2 Forward and reverse primer sequences, lengths of the PCR products and annealing temperatures of the investigated genes used for PCR-DNA sequencing
Gene | Forward | Reverse | Annealing temperature (°C) | Length of PCR product (bp) | Reference |
|---|---|---|---|---|---|
IL-1α | 5′-ATGGCCAAAGTCCCTGACCTC-3′ | 5′-ATGTAGCAGCCGTCATGTATGG-3′ | 60 | 472 | Current study |
IL-1β | 5′-GAACCTTCATTGCCCAGGTTTC − 3′ | 5′-ACTGACTGCACGGCTGCATCA − 3′ | 58 | 388 | Current study |
IL-6 | 5′-GAGAGCTCCATCAGCCCTCCAG-3′ | 5′-GGAGTGGTTATTAGACCTGCGA-3′ | 62 | 526 | Current study |
TNF-α | 5′-CAGACCAAGGTCAACATCCTCT-3′ | 5′-CAGGCCTCACTTCCCTACATC-3′ | 62 | 388 | Current study |
IL-10 | 5′-ACAACAGGGGCTTGCTCTTGC-3′ | 5′-CTCTCTTGGAGCATATTGAAGAC-3′ | 60 | 526 | Current study |
LFA-1 | 5′-TTCCTACACCATCGTGATGAG-3′ | 5′- ATACATTCCTGATAACCAGGGT-3′ | 60 | 438 | Current study |
CR2 | 5′-TAGCAGCTTAGGGACAGCTCT-3′ | 5′-CTATTGTAATATTCACAGATA-3′ | 58 | 381 | Current study |
IL-17 | 5′-ATGGCGTCTATGAGAACTGCCT-3′ | 5′-TTAAGCCACATGGCGGACAAT-3′ | 62 | 462 | Current study |
IL-13 | 5′-CTTAGGCCAGCCTATGCGTCTG-3′ | 5′- TGGTGTCTCGGACGTACTCACT-3′ | 58 | 376 | Current study |
DEFB123 | 5′- CAGAGCACTCCACCATTAGCT − 3′ | 5′- TTATTGATAGAGCTCAAGAATC − 3′ | 62 | 371 | Current study |
SCART1 | 5′-CAGAGCACTCCACCATTAGCT-3′ | 5′- GGCTCTGTCCCCACGCAGTGC-3′ | 62 | 220 | Current study |
ICAM1 | 5′-CGCCTCCGCCTGCGATGGCTC-3′ | 5′- CTTCACCCACAGGCTGCCAGAGT-3′ | 60 | 399 | Current study |
SOD1 | 5′-GCGTCGGCGTGTGTTCTGCGG-3′ | 5′-ATCTACGATATCCACAATGGCA-3′ | 62 | 387 | Current study |
CAT | 5′-TCTGCAGCGCCGCTCAGACAC-3′ | 5′-TGGAGTATCTGGTAATGTCATG-3′ | 60 | 308 | Current study |
GPX1 | 5′-AGCTCACTGCTCTCAACTTGGA-3′ | 5′- ACGTGATGAACTTAGGGTCGGT − 3′ | 60 | 494 | Current study |
NOS | 5′-GGCACGAGCGCCCGTCCGCCC-3′ | 5′-TCGCTCTCTCGGAGGTGGTAG-3′ | 58 | 540 | Current study |
HMOX1 | 5′-AGCACTGGTGACACCTTCTAG-3′ | 5′- TCGGCCACAATATCTGGGCTC-3′ | 62 | 387 | Current study |
NQO1 | 5′-CTACGCAGCCGCAGGATGGAGC-3′ | 5′- GCTCCATCCTGCGGCTGCGTAGAACAT-3′ | 64 | 377 | Current study |