Table 3 Oligonucleotide primer sequences, accession numbers, annealing temperatures and PCR product sizes of the investigated genes via real-time PCR
Gene | Primer | Product length (bp) | Annealing Temperature (°C) | Accession number | Source |
|---|---|---|---|---|---|
IL-1α | F5′- CCATACATGACGGCTGCTACA − 3 R5′- TGTCTCAGGCATCTCCTTATGC − 3′ | 180 | 62 | NM_001009808.1 | Current study |
IL-1β | F5′- ACAGATGAAGAGCTGCACCC − 3′ R5′- AGACATGTTCGTAGGCACGG − 3′ | 161 | 58 | NM_001009465.2 | Current study |
IL-6 | F5- TGCAGTCCTCAAACGAGTGG − 3′ R5′- CCGCAGCTACTTCATCCGAA − 3′ | 110 | 62 | NM_001009392.1 | Current study |
TNF-α | F5′- CTTTGGGATCATCGCCCTGT − 3′ R5′- CAGCCCTGAGCCCCTAATTC − 3′ | 129 | 60 | NM_001024860.1 | Current study |
IL-10 | F5′- CTCGACTAGACTCCCCGACA − 3′ R5′- ACTCTAGGGGAGAGGCACAG − 3′ | 102 | 58 | NM_001009327.1 | Current study |
LFA-1 | F5′- TTGGGTACCGTGTTCTGCAA − 3′ R5′- TCACATGTTCGAGACAGCCC − 3′ | 223 | 58 | NM_001035124.1 | Current study |
CR2 | F5′- TACCCTAGAAGGCAGTCCCC-3′ R5′- AAGGTGACAACACCCAAGCA G − 3′ | 153 | 60 | NM_001009724.1 | Current study |
IL-17 | F5′- TTATCACAAGCGCTCCACCT − 3′ R5′- GTGATGGTCCACCTTCCCTT − 3′ | 133 | 58 | XM_004018887.5 | Current study |
IL-13 | F5′- TCCAGCACTAAAGCAGTGGG − 3′ R5′- TGCTGGCTGTCAGACAAGAG − 3′ | 141 | 62 | NM_001082594.1 | Current study |
DEFB123 | F5′- ACTGTGCTGTGTGAAGTCCA − 3′ R5′- GGGGTTAGACTCAGGGTAGC − 3′ | 112 | 58 | XM_027976443.1 | Current study |
SCART1 | F5′- CCACTGGGACTTGGCAGAC − 3′ R5′- GCTCTGTCCCCACGCA − 3′ | 140 | 58 | EF215856.1 | Current study |
ICAM1 | F5′- CACCATATACTGGTTCCCGGAG − 3 R5′- CATGGTCCTCTCTTCTCGGC − 3′ | 224 | 62 | NM_001009731.1 | Current study |
SOD1 | F5- TGATCATGGGTTCCACGTCC − 3′ R5′- CACATTGCCCAGGTCTCCAA − 3′ | 139 | 60 | NM_001145185.2 | Current study |
CAT | F5- TTCGCTTCTCCACTGTTGCT − 3′ R5′- CCGGATCCTTCAGGTGTGTC − 3′ | 207 | 58 | XM_004016396.5 | Current study |
GPX1 | F5- AACGTAGCATCGCTCTGAGG − 3 R5- CAAACTGGTTGCACGGGAAG − 3 | 115 | 62 | XM_004018462.5 | Current study |
NOS | F5′- CTCCCAAGGTGACTTCCCAGA − 3′ R5′- ACCAAAGGGCTGACTGTAGG − 3′ | 142 | 58 | NM_001129901.1 | Current study |
HMOX1 | F5′- CAAGCGCTATGTTCAGCGAC − 3′ R5′- GCTTGAACTTGGTGGCACTG − 3′ | 206 | 60 | MK630326.1 | Current study |
NQO1 | F5′- GGCAGTGGCTCCATGTACTC − 3′ R5′- TAGATGGCCACCTCACATGC − 3′ | 116 | 62 | XM_012190046.4 | Current study |
ß. actin | F5′- GCAAAGACCTCTACGCCAAC-3′ R5′- TGATCTTCATCGTGCTGGGT − 3′ | 114 | 60 | NM_001009784.3 | Current study |